COURSE DESCRIPTION - This lecture/laboratory course will provide students with a solid understanding of basic sterile cell culture techniques through the growth and maintenance of both normal and transformed adherent and suspension cell in culture.   Topics include primary and explant culture techniques, trypsinization and media supplementation, cell counting, determining viability and growth curves in plate and well cultures, single cell cloning, transfection technologies, fluorescent analysis, photomicrography, and reporter assays, cryopreservation, cell cycle determinants and the induction of apoptosis.  This course is strongly recommended for those students who plan to do cell or tissue work in cancer or neuroscience research for their senior project.

PREREQUISITES - Principles of Biology I and II; Histology or Molecular Cell Biology is strongly recommended.

GOAL: The main goal of this one-semester laboratory course is the mastery of basic sterile techniques and assays that will culminate in the design and implementation of a research project to be qualitatively and quantitatively analyzed for oral and written presentation.

TEXT: CULTURE OF ANIMAL CELLS: A MANUAL OF BASIC TECHNIQUE, 5e. 2007. Freshney, R. I.,  John Wiley & Sons, Inc., New Jersey.

READINGS: Chapters from the assigned text MUST be read before the appropriate lectures. Outlines for each chapter may be given to aid in the study of the material covered.  Supplemental protocols will be provided on Blackboard.

STUDENT ASSESSMENT: 35% = LABORATORY/PROJECT ASSESSMENT 25% = 2 SCHEDULED EXAMINATIONS** (short answer, theory exams) 20% = PROJECT PRESENTATION (Powerpoint) 15% = LABORATORY REPORT (journal article format) 5% = PORTFOLIO SUBMISSION

** NO MAKEUP EXAMS WILL BE GIVEN

8/29/07

Design and Layout
 Equipment

9/05/07

Aseptic Technique
 Safety
 Culture Vessels

9/12/07

Media
 Serum-Free Media
 Preparation and Sterilization

9/19/07

Primary Culture
 Cell Lines

9/26/07

Cryopreservation 

10/03/07

EXAM I

10/10/07

Cloning and Selection
 Cell Separation

10/17/07

Characterization
 Differentiation

10/24/07

Transformation
 Contamination

10/31/07

Quantitation
 Cytotoxicity

11/07/07

Specialized Cells
 Tumor Cells
 Scale-Up

11/14/07

PROJECT PRESENTATIONS

11/28/07

CLEAN UP

12/05/07

EXAM II

12/05/07

BIOLOGY DEPARTMENT HONOR CODE

The biology department has adopted a “zero-tolerance” policy regarding any form of academic dishonesty.
Please read the guidelines at http://www.mville.edu/biology/honorcode.html.
As a student enrolled in this Biology course at Manhattanville College, you must agree to follow
the honor code of conduct as outlined and indicate so in Blackboard under Course Information.