COURSE DESCRIPTION - This one-semester laboratory/lecture course will acquaint the student with both the scanning electron microscope (SEM) and the transmission electron microscope (TEM) as tools for research and education. Practical applications will be stressed, and students will have the opportunity to choose the most appropriate techniques for their needs. Included among these skills are microscope usage and maintenance, specimen preparation, ultrasectioning, staining techniques, photographic skills, and evaluation and presentation of micrographs.

3

2/06/04

5

2/13/04

5

2/20/04

7

3/05/04

8

3/19/04

10

4/02/04

13

4/16/04

15

5/05/04

1

1/23/04

OVERVIEW

2

1/30/04

TISSUE PREPARATION

• SEM samples to buffer
• TEM samples to resin

3

2/06/04

SEM SAMPLE PREPARATION

• Critical point drying
• Mounting samples on stubs
• Sputter-coating samples

4

2/13/04

JEOL T-20 SCANNING ELECTRON MICROSCOPE

• Theory and routine use
• Exposing Polaroid film

5

2/20/04

ULTRAMICROTOMY FOR TEM

• Knives
• Specimen blocks, Trimming

6

2/27/04

ULTRAMICROTOMY FOR TEM

• Use of ultratome
• Thick sections

7

3/05/04

STAINING TECHNIQUES

• Thin sections
• Counterstaining grids

8

3/19/04

HITACHI HS-8 TRANSMISSION EM

• Theory and routine use
• Photomicrography

9

3/26/04

DARKROOM TECHNIQUES

• Development of negatives
• Printing

10

4/23/04

ANALYSIS OF MICROGRAPHS