 |
||||
COURSE DESCRIPTION - This course studies the microscopic anatomy of animal tissues and organs as elucidated by brightfield microscopy. The laboratory also encompasses a broad range of cytological and microtechnique procedures. PREREQUISITES - Principles of Biology I and II GOAL: to prepare mammalian tissues and stain slides using cytological, histochemical, and immunocytochemical techniques and to study human microscopic anatomy of these tissues and commercial preparations. TEXTS: HISTOLOGY: A TEXT AND ATLAS, 2005, 5th ed., Ross, M.H. and Pawlina, W., Lippincott/Williams and Wilkins, Philadelphia, PA.COLOR ATLAS OF BASIC HISTOLOGY, 2003, 3rd ed., Berman, I., Lange Medical Books/McGraw Hill, New York. STUDENT ASSESSMENT: 50% HISTOLOGY
50% HISTOTECHNIQUE LAB
|
HISTOLOGY LABORATORY SYLLABUS
| Topics | Lab Manual Pages | Dates |
|---|---|---|
| Check In Buccal smear - Aceto orcein Sputum smear - Concentration method |
4-5 |
8/25/08 |
| Dissect and fix tissue | 9-12 |
8/28/08 |
| Embedding, Microtoming | 13-17 |
9/04/08 |
| Blood and bone
marrow smears - Wright’s blood stain |
8 |
9/08/08 |
| Papanicolaou stain - concentrated sputum prep - fresh sputum prep |
6-7 |
9/11/08 |
| Harris’ Hematoxylin and Eosin - Regressive Method | 18-27 |
9/15/08 |
| Weigert’s Iron Hematoxylin - Progressive Method | 27 |
9/18/08 |
| Hematoxylin,
Modified Masson’s CT stain (Aniline blue, Light green, Metanil yellow) |
28-29 |
9/22/08-9/25/08 |
| Miller's Elastic Stain | 30 |
9/29/08 |
| Gomori Iron Reaction |
31-33 |
10/02/08 |
| Prepared Slides Repeat Stains if necessary |
|
10/06/08 |
| Frozen Sections |
|
10/14/08 |
| Immunocytochemistry - Anti-Pan Cytokeratin | 36-38 |
10/16/08 |
| Immunocytochemistry - Anti-Vimentin | 36-38 |
10/20/08 |
| PRACTICAL EXAM I - TISSUES | List 1 |
10/23/08 |
| Immunocytochemistry -Anti-a-Actin (Smooth Muscle) | 36-38 |
10/27/08 |
| Immunocytochemistry - Anti-GFAP | 36-38 |
10/30/08 |
| Gomori's Hexamine Stain for Basement Membrane | 34 |
11/03/08 |
| Rapid PTAH stain for astrocytes | 35 |
11/06/08 |
| Prepared Slides Repeat Stains if necessary |
|
11/10/08 |
| Metachromatic Staining - Toluidine blue | 40 |
11/13/08 |
| Cartilage stain - Safranine O/Fast Green | 41 |
11/17/08 |
| Cartilage stain - Alcian blue/PAS | 39, 42 |
11/20/08 |
| Periodic Acid/Schiff Reagent (PAS ) - Modifications | 39, 43 |
11/24/08 |
| Submit slides - Clean up! | 46 |
12/01/08 |
| PRACTICAL EXAM II - SYSTEMS |
List 2 |
12/04/08 |
|
|
Lecture
Outlines|
|
Dr.
Annemarie Bettica - X5120 Brownson Hall, Rm. 225 betticaa@mville.edu |
|
|
Last Update:
07/14/08
Web Author: Dr. Annemarie Bettica
Copyright © 2008 by A. Bettica - ALL RIGHTS RESERVED