The purpose of this project is to test various peptide samples isolated from Phormia regina, in order to identify and determine their ability to inhibit microbe growth as compared to known controls. A toxicity assay will also help to determine the effectiveness of peptide isolates and to identify if reagents used to purify peptide samples toxicity were the direct cause of inhibition against pathogenic bacterial strains. Peptides will be isolated from the P. regina blowfly utilizing the Bio-RadR Ready Prep protein extraction kit, a commercial protein purification process. The purpose of the protein purification is to isolate peptides and determine the toxicity of purification reagents before suggested for usage to treat human eye diseases. Anticipated and potential problems include the presence of acute toxicity in animal tissue samples. LD50 of each purification reagent and Phormia regina peptides are determining factors in use of P. regina peptides for medicinal purposes to combat conjunctivital bacterial growth as seen in ocular and epithelial tissues. If peptides illustrate minimal toxicity to target tissues, with further studies these samples could be used as treatments against bacterial infections of the eye such as ocular herpes and bacterial conjunctivitis. However, if peptide samples contain high toxicity levels a new method of purification will need to be identified.